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Fujian Medical University, China [09:30AM-10:00AM
Title: Causal Effect of Gut Microbiota on DNA Methylation Phenotypic Age Acceleration: A Two-Sample Mendelian Randomization Study
Background: The causal relationship between gut microbiota and DNA methylation phenotypic age acceleration remains unclear. This study aims to examine the causal effect of gut microbiota on the acceleration of DNA methylation phenotypic age using Mendelian randomization analysis.
Methods: A total of 212 gut microbiota species were included in this study, and their 16S rRNA sequencing data were obtained from the Genome-wide Association Study (GWAS) database (http://gwas.mrcieu.ac.uk/datasets/). The GWAS data corresponding to DNA methylation phenotypic age acceleration were selected as the outcome variable for this study. Two-sample Mendelian randomization (TSMR) analysis was conducted using R software, and a scatter plot was generated to visualize the results. During the analysis process, careful consideration was given to address potential biases arising from linkage disequilibrium and weak instrumental variables. Furthermore, rigorous assessments for heterogeneity and horizontal pleiotropy were conducted to ensure the robustness of the findings.
Results: The results from inverse-variance weighting (IVW) analysis revealed significant associations (P < 0.05) between single nucleotide polymorphisms (SNPs) corresponding to 16 gut microbiota species and DNA methylation phenotypic age acceleration. Out of the total, 12 gut microbiota species exhibited consistent and robust causal effects, as indicated by stable SNP associations, and were thoroughly validated through rigorous assessments for heterogeneity and horizontal pleiotropy. Among the 12 investigated gut microbiota species, 7 displayed a significant positive correlation with the outcome, indicating a positive causal effect on the DNA methylation phenotypic age acceleration. Conversely, 5 species showed a significant negative correlation with the outcome, suggesting a negative causal effect on DNA methylation phenotypic age acceleration.
Conclusion: This study utilized Mendelian randomization analysis to unravel the intricate causal effects of various gut microbiota species on DNA methylation phenotypic age acceleration.
Second-year M.D. student in Oncology, Fujian Medical University and Resident Physician